Keynote

Gerben Zijlstra

Global Technology Consultant Intensified & Continuous Biomanufacturing at Sartorius Stedim Biotech

Premium Lecture

Joris van de Velde

Senior Application Engineer at Separation and Purification Sciences Division at 3M

Transition to Next Gen Downstream Processing with Single Use Polishing AEX Membrane Chromatography

Utilisation of a new guanidinium-ligand allows replacing resin-based AEX chromatography columns by compact single-use capsules. 
This technology overcomes many of the current downstream challenges by offering robust HCP, DNA and viral clearance in a wide range of pH, conductivity and buffers, while ensuring excellent target protein recoveries. 
Topics covered in this session include:
-    Single use AEX chromatography
-    Host cell Protein and Host cell DNA clearance
-    Viral clearance
-    Downstream process optimization and intensification

Parallel session 1

Nico Oosterhuis

Director Technology at Celltainer Biotech

Single-use bioreactors for cellular agriculture application

Cellular agriculture is a relatively new field of bioprocessing. Cultivation of mammalian-, plant- and insect cells is known and applied over decades already, but their use is limited to production of mostly biopharmaceuticals. Since the introduction of the “cultured meat burger” by Mosa Meat BV in 2013, many attempts are currently being carried out to produce cultured meat on an economical way. 
Application of plant cells to produce secondary metabolites, cacao products or cultivating somatic embryonic plant cells for growing trees, becomes more important and feasible.
Single-use bioreactors developed for the production of biopharmaceuticals can serve as a basis for a dedicated bioreactor design for such products as well. These reactor types may not fully address the capacity need of cellular agricultural products, though, by choosing a scale-out approach, single-use bioreactors, due to their flexibility, can contribute to get cellular agricultural production feasible. 
Developing systems for cellular agriculture, will require more than adopting the stirred tank reactors that are commonly used for mammalian cells, and that more sophisticated reactor designs are likely to be needed to achieve optimal conditions and minimal cost of goods.
 

Patrick Hoevenaars

Sales Manager Benelux, Continuous Monitoring Systems and Idustrial Measurements at Vaisala Oyj

Humidity measurement in H2O2 bio-decontamination – Relative saturation as the key

•    Basics of bio-decontamination with vaporized H2O2
•    The importance of continious measurement during bio-decontamintaion
•    Patented PEROXCAP technology
•    The difference between Relative Humidity  (%RH) and Relative Saturation (RS%)
•    Five most typical pitfalls in humidity measurement during bio-decontamination

Parallel session 2

Parallel session 3

Ralph Daumke

Global Market Manager Life Science at Filtrox AG

Using Diatomaceous earth (DE) and FILTRODISC™ BIO SD for Biotechnology processes

The presentation will describe an economical, efficient and robust technology for clarification of fermentation broth and/or cell debris solutions for the production of e.g. mAb`s or plasmid DNA and viral vectors. By using Diatomaceous earth and FILTRODISC™ BIO SD it is possible to replace the centrifuge step and establish a fast and reliable single-use technology in the biotech process. FILTRODISC™ BIO SD is linear scalable from process development to production. The production scale fits to all common single-use connectors on the market and can be used as an integrator of different single-use systems.  

Parallel session 4

Keynote